Gene Mutants - Detail

ME No. ME9498
Strain Name AQ8948
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 pro ilv metB1 thyA recA200 deoB (or deoC) rnhA224 tus::kan dnaA850::Tn10
Parent Donor AQ3519
Parent Recipient AQ8931
Methods TD./PI
Latest Marker dnaA850::Tn10
Selection Tc-r
Source
Gene Mutants Detail Info
Reference Kogoma T, Barnard KG, Hong X.
RecA, Tus protein and constitutive stable DNA replication in Escherichia coli rnhA mutants.
Mol Gen Genet  (1994)  244(5)   557-62  
[PubMed ID = 8078483]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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