Gene Mutants - Detail

ME No. ME9490
Strain Name AQ8885
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 thyA deoB (or deoC) metD88 lac-del3 ilv dnaA5 recA200 polA25::spc zag::dTn10(Tc-s) malF3089::Tn10
Parent Donor AQ6471
Parent Recipient AQ8877
Methods TD./PI
Latest Marker Km-s, lexA+
Selection Tc-r
Source
Gene Mutants Detail Info
Reference Cao Y, Rowland RR, Kogoma T.
DNA polymerase I and the bypassing of RecA dependence of constitutive stable DNA replication in Escherichia coli rnhA mutants.
J Bacteriol  (1993)  175(22)   7247-53  
[PubMed ID = 8226671]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Ts
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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