遺伝子変異株 - 詳細

ME No. ME9489
菌株名 AQ8882
性別 F-
組み込みプラスミド
自律複製プラスミド
プロファージ
遺伝子型 metE90 trpA9605 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 sfiA11 zih-35::dTn10(Tc-s)
親株(供与菌) AQ6375
親株(受容菌) AQ8869
変異導入方法 CROSS
最終選別マーカー UVr, recA+
選別マーカー Thy+
入手源
ゲノムマップ
文献 Cao Y, Kogoma T.
The mechanism of recA polA lethality: suppression by RecA-independent recombination repair activated by the lexA(Def) mutation in Escherichia coli.
Genetics  (1995)  139(4)   1483-94  
[PubMed ID = 7789754]  [RRC文献情報] 
培地条件 Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
その他備考 Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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