Gene Mutants - Detail

ME No. ME9482
Strain Name AQ8865
Sex F+
Plasmid Integrated
Plasmid Autonomous F'
Prophage
Genotype hsdR514(r- m+) supE44 supF58 lacY1 or Δ(lacIZY)6 galK2 galT22 metB1 trpR55
Parent Donor
Parent Recipient
Methods
Latest Marker
Selection
Source
Gene Mutants Detail Info
Reference Cao Y, Kogoma T.
Requirement for the polymerization and 5'-->3' exonuclease activities of DNA polymerase I in initiation of DNA replication at oriK sites in the absence of RecA in Escherichia coli rnhA mutants.
J Bacteriol  (1993)  175(22)   7254-9  
[PubMed ID = 8226672]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), chloramphenicol (25μg/ml).
Other Remarks same as RM2428 (CJ251): Lifsics et al., 1992, J. Bacteriol., 174, 6965-6973.
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