Gene Mutants - Detail

ME No. ME9477
Strain Name AQ8851
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 pro ilv metB1 thyA deoB (or deoC) priA1::kan
Parent Donor AQ8845
Parent Recipient AQ634
Methods TD./PI
Latest Marker filamentous, priA1
Selection Km-r
Source
Gene Mutants Detail Info
Reference Masai H, Asai T, Kubota Y, Arai K, Kogoma T.
Escherichia coli PriA protein is essential for inducible and constitutive stable DNA replication.
EMBO J  (1994)  13(22)   5338-45  
[PubMed ID = 7525276]  [RRC reference] 
Mizukoshi T, Tanaka T, Arai K, Kohda D, Masai H.
A critical role of the 3' terminus of nascent DNA chains in recognition of stalled replication forks.
J Biol Chem  (2003)  278(43)   42234-9  
[PubMed ID = 12917421]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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