Gene Mutants - Detail

ME No. ME9476
Strain Name AQ8850
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpR trpA9605(Am) his-29 (Am) ilv pro-2 arg427 thyA deoB or C tsx IN(rrnD-rrnE)1 lacY(?) tus::kan
Parent Donor
Parent Recipient
Methods
Latest Marker
Selection
Source from T. Hill.
Reference Kogoma T, Barnard KG, Hong X.
RecA, Tus protein and constitutive stable DNA replication in Escherichia coli rnhA mutants.
Mol Gen Genet  (1994)  244(5)   557-62  
[PubMed ID = 8078483]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks same as TH210
Request
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