遺伝子変異株 - 詳細

ME No. ME9472
菌株名 AQ8817
性別 F-
組み込みプラスミド
自律複製プラスミド
プロファージ
遺伝子型 metE90 trpA9605lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 recA200 lexA71::Tn5 sfiA11 polA25::spc zih-35::dTn10(Tc-s) tnaA::dTn10(Tc-s) dnaA5
親株(供与菌)
親株(受容菌) AQ8747
変異導入方法 OTHER METHODSBochner selection
最終選別マーカー tnaA::dTn10(Tc-s)
選別マーカー Tc-s
入手源
ゲノムマップ
文献 Cao Y, Rowland RR, Kogoma T.
DNA polymerase I and the bypassing of RecA dependence of constitutive stable DNA replication in Escherichia coli rnhA mutants.
J Bacteriol  (1993)  175(22)   7247-53  
[PubMed ID = 8226671]  [RRC文献情報] 
培地条件 Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
その他備考 Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
分譲依頼
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