Gene Mutants - Detail

ME No. ME9468
Strain Name AQ8720
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype thr-1 leuB6 Δ(gpt-proA)62 his-4 argE3 thi-1 rpsL31 galK2 lac+ ilv(ts) sulA221 lexA71::Tn5 malF3089::Tn10 recA441 ara-14 lac xyl-5 mtl-1 tsx-33 supE44
Parent Donor AQ6471
Parent Recipient AQ4414
Methods TD./PI
Latest Marker lexA71::Tn5 malF3089::Tn10
Selection TC-r, Km-r
Source
Reference Cao Y, Kogoma T.
The mechanism of recA polA lethality: suppression by RecA-independent recombination repair activated by the lexA(Def) mutation in Escherichia coli.
Genetics  (1995)  139(4)   1483-94  
[PubMed ID = 7789754]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml).
Other Remarks Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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