Gene Mutants - Detail

ME No. ME9456
Strain Name AQ8622
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage λ+ λp[sfiA::lac cI(Ind-)]
Genotype pro Δ(pro-lac) rpsL λp[sfiA::lac cI(Ind-)] rnhA339::cat rpoB3595
Parent Donor AQ8580
Parent Recipient AQ8136
Methods TD./PI
Latest Marker Rif-r, rpoB3595
Selection Tc-r
Source
Gene Mutants Detail Info
Reference Kogoma T.
Escherichia coli RNA polymerase mutants that enhance or diminish the SOS response constitutively expressed in the absence of RNase HI activity.
J Bacteriol  (1994)  176(5)   1521-3  
[PubMed ID = 8113195]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), chloramphenicol (25μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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