Gene Mutants - Detail

ME No. ME9450
Strain Name AQ8560
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 pro ilv metB1 thyA deoB(or deoC) polA25::spc zih-35::Tn10
Parent Donor AQ8534
Parent Recipient AQ634
Methods TD./PI
Latest Marker UVs, polA25
Selection Tc-r
Source
Gene Mutants Detail Info
Reference Cao Y, Rowland RR, Kogoma T.
DNA polymerase I and the bypassing of RecA dependence of constitutive stable DNA replication in Escherichia coli rnhA mutants.
J Bacteriol  (1993)  175(22)   7247-53  
[PubMed ID = 8226671]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), spectinomycin (40μg/ml).
Other Remarks Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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