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Gene Mutants - Detail

ME No. ME9443
Strain Name AQ8501
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage λ+ λp[sfiA::lac cI(Ind-)]
Genotype pro Δ(pro-lac) rpsL λp[sfiA::lac cI(Ind-)] rnhA339::cat rpoB5501
Parent Donor
Parent Recipient AQ8136
Methods MUTAGENESIS/OthersEMS
Latest Marker rpoB5501
Selection Rif-r
Source
Reference Kogoma T, Hong X, Cadwell GW, Barnard KG, Asai T.
Requirement of homologous recombination functions for viability of the Escherichia coli cell that lacks RNase HI and exonuclease V activities.
Biochimie  (1993)  75(1-2)   89-99  
[PubMed ID = 8389213]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), chloramphenicol (25μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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