Gene Mutants - Detail

ME No. ME9428
Strain Name AQ8326
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 pro ilv metB1 thyA deoB (or deoC) recJ284::dTn10(Tc-s) rnhA224 zag::Tn10
Parent Donor AQ978
Parent Recipient AQ8022
Methods TD./PI
Latest Marker Ls, rnhA224
Selection Tc-r
Source
Reference Kogoma T, Barnard KG, Hong X.
RecA, Tus protein and constitutive stable DNA replication in Escherichia coli rnhA mutants.
Mol. Gen. Genet.  (1994)  244(5)   557-62  
[PubMed ID = 8078483]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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