Gene Mutants - Detail

ME No. ME9428
Strain Name AQ8326
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 pro ilv metB1 thyA deoB (or deoC) recJ284::dTn10(Tc-s) rnhA224 zag::Tn10
Parent Donor AQ978
Parent Recipient AQ8022
Methods TD./PI
Latest Marker Ls, rnhA224
Selection Tc-r
Source
Gene Mutants Detail Info
Reference Kogoma T, Barnard KG, Hong X.
RecA, Tus protein and constitutive stable DNA replication in Escherichia coli rnhA mutants.
Mol Gen Genet  (1994)  244(5)   557-62  
[PubMed ID = 8078483]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
Request
page-top
/ecoli/strain