遺伝子変異株 - 詳細

ME No. ME9425
菌株名 AQ8273
性別 F-
組み込みプラスミド
自律複製プラスミド
プロファージ λ+ λp[sfiA::lac cI(Ind-)]
遺伝子型 pro Δ(pro-lac) rpsL λp[sfiA::lac cI(Ind-)] rnhA224 recD1903::miniTc
親株(供与菌)
親株(受容菌)
変異導入方法
最終選別マーカー T4 gene2 sensitive, recD1903
選別マーカー Tc-r
入手源
ゲノムマップ
文献 Kogoma T, Hong X, Cadwell GW, Barnard KG, Asai T.
Requirement of homologous recombination functions for viability of the Escherichia coli cell that lacks RNase HI and exonuclease V activities.
Biochimie  (1993)  75(1-2)   89-99  
[PubMed ID = 8389213]  [RRC文献情報] 
培地条件 Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml).
その他備考 Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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