Gene Mutants - Detail

ME No. ME9422
Strain Name AQ8224
Sex F-
Plasmid Integrated
Plasmid Autonomous
Genotype recN1502::Tn5 his-4 argE3 leuB6 proA2 thr-1 thi-1 rpsL31 galK2 lacY1 ara14 xyl5 mtl-1 kdg51 supE44 tsx-33
Parent Donor
Parent Recipient
Latest Marker
Reference Kogoma T, Cadwell GW, Barnard KG, Asai T.
The DNA replication priming protein, PriA, is required for homologous recombination and double-strand break repair.
J Bacteriol  (1996)  178(5)   1258-64  
[PubMed ID = 8631700]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks same as RDK1041: Lovett et al., 1988, Genetics, 120, 37-45.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.