Gene Mutants - Detail

ME No. ME9422
Strain Name AQ8224
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype recN1502::Tn5 his-4 argE3 leuB6 proA2 thr-1 thi-1 rpsL31 galK2 lacY1 ara14 xyl5 mtl-1 kdg51 supE44 tsx-33
Parent Donor
Parent Recipient
Methods
Latest Marker
Selection
Source
Reference Kogoma T, Cadwell GW, Barnard KG, Asai T.
The DNA replication priming protein, PriA, is required for homologous recombination and double-strand break repair.
J Bacteriol  (1996)  178(5)   1258-64  
[PubMed ID = 8631700]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks same as RDK1041: Lovett et al., 1988, Genetics, 120, 37-45.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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