Gene Mutants - Detail

ME No. ME9409
Strain Name AQ8115
Sex F-
Plasmid Integrated
Plasmid Autonomous Other
Prophage λ+ λp[sfiA::lacZ+ cI(Ind-)]
Genotype Δ(srl-recA)306 thr-1 leuB6 his4 argE3 ilv(ts) Δlac λp[sfiA::lacZ+ cI(Ind-)] supE44 sfiA211 galK2 ara-15 xyl-5 mtl-1 srl::Tn10 tsx-33 rpsL31 thyA deoB(or C)
Parent Donor
Parent Recipient AQ8014
Methods TF.
Latest Marker miniF-recA+
Selection Km-r
Reference Asai T, Sommer S, Bailone A, Kogoma T.
Homologous recombination-dependent initiation of DNA replication from DNA damage-inducible origins in Escherichia coli.
EMBO J  (1993)  12(8)   3287-95  
[PubMed ID = 8344265]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml).
Other Remarks Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.