Gene Mutants - Detail

ME No. ME9403
Strain Name AQ8072
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 pro ilv metB1 thyA deoB(or C) recB21 zga::Tn10
Parent Donor AQ8045
Parent Recipient AQ634
Methods TD./PI
Latest Marker UV-s, recB21
Selection Tc-r
Source
Gene Mutants Detail Info
Reference Kogoma T, Hong X, Cadwell GW, Barnard KG, Asai T.
Requirement of homologous recombination functions for viability of the Escherichia coli cell that lacks RNase HI and exonuclease V activities.
Biochimie  (1993)  75(1-2)   89-99  
[PubMed ID = 8389213]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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