遺伝子変異株 - 詳細

ME No. ME9399
菌株名 AQ8046
性別 F-
組み込みプラスミド
自律複製プラスミド
プロファージ
遺伝子型 trpA9605 his-29 pro ilv metB1 thyA deoB(or C) rnhA339::cat recB270(Ts) zga::Tn10
親株(供与菌) AQ4432
親株(受容菌) AQ8033
変異導入方法 TD./PI
最終選別マーカー UVts, recB270
選別マーカー Tc-r
入手源
ゲノムマップ
文献 Kogoma T, Hong X, Cadwell GW, Barnard KG, Asai T.
Requirement of homologous recombination functions for viability of the Escherichia coli cell that lacks RNase HI and exonuclease V activities.
Biochimie  (1993)  75(1-2)   89-99  
[PubMed ID = 8389213]  [RRC文献情報] 
培地条件 Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
その他備考 Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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