Gene Mutants - Detail

ME No. ME9395
Strain Name AQ8022
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 proB(or C) ilv metB1 thyA deoB(or C) recJ284::dTn10(Tc-s)
Parent Donor
Parent Recipient AQ3362
Methods OTHER METHODSBochner selection
Latest Marker recJ284::dTn10(Tc-s)
Selection Tc-(Bochner selection)
Source
Gene Mutants Detail Info
Reference Kogoma T, Hong X, Cadwell GW, Barnard KG, Asai T.
Requirement of homologous recombination functions for viability of the Escherichia coli cell that lacks RNase HI and exonuclease V activities.
Biochimie  (1993)  75(1-2)   89-99  
[PubMed ID = 8389213]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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