遺伝子変異株 - 詳細

ME No. ME9394
菌株名 AQ8017
性別 F-
組み込みプラスミド
自律複製プラスミド
プロファージ
遺伝子型 trpA9605 his-29 pro ilv metB1 thyA deoB(or deoC) zga::Tn10 recB21
親株(供与菌)
親株(受容菌) AQ5931
変異導入方法 SPONTANEOUS
最終選別マーカー thyA
選別マーカー Thy-(trimethoprim selection)
入手源
ゲノムマップ
文献 Hong X, Cadwell GW, Kogoma T.
Activation of stable DNA replication in rapidly growing Escherichia coli at the time of entry to stationary phase.
Mol Microbiol  (1996)  21(5)   953-61  
[PubMed ID = 8885266]  [RRC文献情報] 
培地条件 Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
その他備考 Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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