Gene Mutants - Detail

ME No. ME9393
Strain Name AQ8014
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage λ+ λp[sfiA::lacZ cI(Ind-)]
Genotype ΔrecA306 thr-1 leuB6 his-4 argE3 ilv(ts) Δlac λp[sfiA::lacZ cI(Ind-)] supE44 sfiA211 galK2 ara-15 xyl-5 mtl-1 srl::Tn10 tsx33 rpsL31 thyA deoB(or C)
Parent Donor
Parent Recipient AQ7982
Methods
Latest Marker
Selection
Source
Gene Mutants Detail Info
Reference Asai T, Sommer S, Bailone A, Kogoma T.
Homologous recombination-dependent initiation of DNA replication from DNA damage-inducible origins in Escherichia coli.
EMBO J  (1993)  12(8)   3287-95  
[PubMed ID = 8344265]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml).
Other Remarks
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