Gene Mutants - Detail

ME No. ME9393
Strain Name AQ8014
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage λ+ λp[sfiA::lacZ cI(Ind-)]
Genotype ΔrecA306 thr-1 leuB6 his-4 argE3 ilv(ts) Δlac λp[sfiA::lacZ cI(Ind-)] supE44 sfiA211 galK2 ara-15 xyl-5 mtl-1 srl::Tn10 tsx33 rpsL31 thyA deoB(or C)
Parent Donor
Parent Recipient AQ7982
Methods
Latest Marker
Selection
Source
Reference Asai T, Sommer S, Bailone A, Kogoma T.
Homologous recombination-dependent initiation of DNA replication from DNA damage-inducible origins in Escherichia coli.
EMBO J  (1993)  12(8)   3287-95  
[PubMed ID = 8344265]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml).
Other Remarks
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