Gene Mutants - Detail

ME No. ME9387
Strain Name AQ7960
Sex F-
Plasmid Integrated
Plasmid Autonomous
Genotype trpA9605 his-29 proB(or C) ilv metB1 thyA deoB(or C) ruvC51 eda51::Tn10
Parent Donor AQ7946
Parent Recipient AQ2634
Methods TD./PI
Latest Marker UV-s, ruvC51
Selection Tc-r
Reference Asai T, Sommer S, Bailone A, Kogoma T.
Homologous recombination-dependent initiation of DNA replication from DNA damage-inducible origins in Escherichia coli.
EMBO J.  (1993)  12(8)   3287-95  
[PubMed ID = 8344265]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.