Gene Mutants - Detail

ME No. ME9385
Strain Name AQ7805
Sex F-
Plasmid Integrated
Plasmid Autonomous
Genotype metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) supE44 thyA deoB(or C) zif::pML31(Kan-r) oriCdel-1071 recD1903::miniTc
Parent Donor AQ7678
Parent Recipient AQ7797
Methods TD./PI
Latest Marker recD1903::miniTc
Selection Tc-r
Reference Asai T, Imai M, Kogoma T.
DNA damage-inducible replication of the Escherichia coli chromosome is initiated at separable sites within the minimal oriC.
J Mol Biol  (1994)  235(5)   1459-69  
[PubMed ID = 8107086]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml).
Other Remarks Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.