Gene Mutants - Detail

ME No. ME9364
Strain Name AQ6226
Sex F-
Plasmid Integrated
Plasmid Autonomous Other
Genotype thr-1 leuB6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL31 tsx-33 supE44 recA281(Con) lexA3(Ind-) srlC300::Tn10
Parent Donor
Parent Recipient JC11867
Methods TF.
Latest Marker
Selection Ap-r
Reference Magee TR, Kogoma T.
Requirement of RecBC enzyme and an elevated level of activated RecA for induced stable DNA replication in Escherichia coli.
J Bacteriol  (1990)  172(4)   1834-9  
[PubMed ID = 2180906]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), ampicillin (40μg/ml).
Other Remarks Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.