Gene Mutants - Detail

ME No. ME9361
Strain Name AQ5937
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype thr-1 leuB6 Δ(gpt-proA)62 his-4 argE3 thi-1 rpsL31 galK2 lac+ ilv(ts) sulA221 lexA71::Tn5 recA441 ara-14 lac xyl-5 mtl-1 tsx-33 supE44 zga::Tn10 recB21
Parent Donor AQ5931
Parent Recipient AQ2125(JM441)
Methods TD./PI
Latest Marker UV-s, Rec-, recB21
Selection Tc-r
Source
Reference Magee TR, Kogoma T.
Requirement of RecBC enzyme and an elevated level of activated RecA for induced stable DNA replication in Escherichia coli.
J Bacteriol  (1990)  172(4)   1834-9  
[PubMed ID = 2180906]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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