Gene Mutants - Detail

ME No. ME9356
Strain Name AQ5496
Sex F-
Plasmid Integrated
Plasmid Autonomous
Genotype metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) supE44 thyA deoB(or C) zga::dTn10(Tc-s) rnhA224 dnaA850::Tn10
Parent Donor AQ3519
Parent Recipient AQ5441
Methods TD./PI
Latest Marker dnaA850::Tn10
Selection Tc-r
Reference Magee TR, Asai T, Malka D, Kogoma T.
DNA damage-inducible origins of DNA replication in Escherichia coli.
EMBO J  (1992)  11(11)   4219-25  
[PubMed ID = 1396602]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.