Gene Mutants - Detail

ME No. ME9355
Strain Name AQ5486
Sex F-
Plasmid Integrated
Plasmid Autonomous
Genotype metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) supE44 dnaA46 tnaA600::Tn10
Parent Donor AQ5425
Parent Recipient AQ3804
Methods TD./PI
Latest Marker Ts, dnaA46
Selection Tc-r
Reference Hong X, Cadwell GW, Kogoma T.
Escherichia coli RecG and RecA proteins in R-loop formation.
EMBO J  (1995)  14(10)   2385-92  
[PubMed ID = 7774596]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks