Gene Mutants - Detail

ME No. ME9328
Strain Name AQ4401
Sex F-
Plasmid Integrated
Plasmid Autonomous
Genotype metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) supE44 thyA deoB(or C) zif::pML31 oriCdel-1071
Parent Donor AQ3788
Parent Recipient AQ4382
Methods TD./PI
Latest Marker oriCdel-1071
Selection Tc-r
Reference Magee TR, Asai T, Malka D, Kogoma T.
DNA damage-inducible origins of DNA replication in Escherichia coli.
EMBO J  (1992)  11(11)   4219-25  
[PubMed ID = 1396602]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks