Gene Mutants - Detail

ME No. ME9325
Strain Name AQ4319
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype thr-1 leu-6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL-31 tsx-33 sup-37 thyA nfo-1::kan relA1 spoT thiA
Parent Donor
Parent Recipient
Methods
Latest Marker
Selection
Source
Reference Kogoma T, Farr SB, Joyce KM, Natvig DO.
Isolation of gene fusions (soi::lacZ) inducible by oxidative stress in Escherichia coli.
Proc Natl Acad Sci U S A  (1988)  85(13)   4799-803  
[PubMed ID = 2838846]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks same as BW540: Cunningham et al., 1986, J. Bacteriol., 168, 1120-1127.
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