Gene Mutants - Detail

ME No. ME9296
Strain Name AQ3819
Sex F-
Plasmid Integrated
Plasmid Autonomous
Genotype metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8(or 9) supE44 zif-90::pML31 dnaA5
Parent Donor AQ3746
Parent Recipient AQ3803
Methods TD./PI
Latest Marker zif-90::pML31
Selection Km-r
Reference Kogoma T, Kline BC.
Integrative suppression of dnaA(Ts) mutations mediated by plasmid F in Escherichia coli is a DnaA-dependent process.
Mol Gen Genet  (1987)  210(2)   262-9  
[PubMed ID = 2830456]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks