Gene Mutants - Detail

ME No. ME9285
Strain Name AQ3804
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype metE46 trp-3 his-4 thi-1 galK2 lacY1(or lacZ4) mtl-1 ara-9 tsx-3 ton-1 rpsL8 (or 9) supE44 dnaA46
Parent Donor
Parent Recipient
Methods
Latest Marker
Selection
Source
Gene Mutants Detail Info
Reference Kogoma T, Kline BC.
Integrative suppression of dnaA(Ts) mutations mediated by plasmid F in Escherichia coli is a DnaA-dependent process.
Mol Gen Genet  (1987)  210(2)   262-9  
[PubMed ID = 2830456]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks same as CM742: Hanesen et al., 1984, Mol. Gen. Genet., 196, 387-396.
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