Gene Mutants - Detail

ME No. ME9281
Strain Name AQ3764
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 proB (or proC) ilv metB1 thyA deoB (or C) rnhA339::cat
Parent Donor AQ3297
Parent Recipient AQ634
Methods TD./PI
Latest Marker rnhA339::cat
Selection Cm-r
Source
Gene Mutants Detail Info
Reference Masai H, Asai T, Kubota Y, Arai K, Kogoma T.
Escherichia coli PriA protein is essential for inducible and constitutive stable DNA replication.
EMBO J  (1994)  13(22)   5338-45  
[PubMed ID = 7525276]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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