Gene Mutants - Detail

ME No. ME9280
Strain Name AQ3746
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 argH thyA deoB (or deoC) proA3 metD88 Δlac3 ilv dnaA5 zif-90::pML31
Parent Donor
Parent Recipient AQ3742
Methods SPONTANEOUS
Latest Marker zif-90::pML31
Selection Tr
Source
Gene Mutants Detail Info
Reference Kogoma T, Kline BC.
Integrative suppression of dnaA(Ts) mutations mediated by plasmid F in Escherichia coli is a DnaA-dependent process.
Mol Gen Genet  (1987)  210(2)   262-9  
[PubMed ID = 2830456]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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