Gene Mutants - Detail

ME No. ME9279
Strain Name AQ3634
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype recB270(Ts) rpsL31 argE3 his-4 thr-1 proA2 thi
Parent Donor
Parent Recipient
Methods
Latest Marker
Selection
Source
Gene Mutants Detail Info
Reference Magee TR, Kogoma T.
Requirement of RecBC enzyme and an elevated level of activated RecA for induced stable DNA replication in Escherichia coli.
J Bacteriol  (1990)  172(4)   1834-9  
[PubMed ID = 2180906]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks same as SK119: Kushner S. R., 1974, J. Bacteriol., 136, 1213-1218.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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