Gene Mutants - Detail

ME No. ME9276
Strain Name AQ3625
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype thr-1 leuB6 thi-1 lacY1 galK2 ara-4 xyl-5 mtl-1 proA2 his-60 argE3 rpsL31 tsx-33 supE44 recB21 recC22 sbcA23
Parent Donor
Parent Recipient
Methods
Latest Marker
Selection
Source from A. J. Clark.
Reference Tambosi R, Liotenberg S, Bourbon ML, Steunou AS, Babot M, Durand A, Kebaili N, Ouchane S.
Silver and Copper Acute Effects on Membrane Proteins and Impact on Photosynthetic and Respiratory Complexes in Bacteria.
MBio  (2018)  9(6)  
[PubMed ID = 30459190]  [RRC reference
Hasegawa H, Suzuki E, Maeda S.
Horizontal Plasmid Transfer by Transformation in Escherichia coli: Environmental Factors and Possible Mechanisms.
Front Microbiol  (2018)  9   2365  
[PubMed ID = 30337917]  [RRC reference
Asai T, Sommer S, Bailone A, Kogoma T.
Homologous recombination-dependent initiation of DNA replication from DNA damage-inducible origins in Escherichia coli.
EMBO J.  (1993)  12(8)   3287-95  
[PubMed ID = 8344265]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks same as JC8679
Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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