Gene Mutants - Detail

ME No. ME9264
Strain Name AQ3408
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 argH thyA Δlac-3 deoB (or deoC) proA3 metD88 tnaA::Tn10 dnaA5
Parent Donor AQ799
Parent Recipient AQ3364
Methods
Latest Marker Rif-s, rpoB+
Selection Met+
Source
Gene Mutants Detail Info
Reference Hong X, Cadwell GW, Kogoma T.
Activation of stable DNA replication in rapidly growing Escherichia coli at the time of entry to stationary phase.
Mol Microbiol  (1996)  21(5)   953-61  
[PubMed ID = 8885266]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks
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