Gene Mutants - Detail

ME No. ME9260
Strain Name AQ3364
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 metB1 argH thyA Δlac-3 rpoB340 deoB (or deoC) proA3 metD88 tnaA::Tn10 dnaA5
Parent Donor TC745
Parent Recipient AQ685
Methods TD./PI
Latest Marker Ts, dnaA5
Selection Tc-r
Source
Gene Mutants Detail Info
Reference Cao Y, Rowland RR, Kogoma T.
DNA polymerase I and the bypassing of RecA dependence of constitutive stable DNA replication in Escherichia coli rnhA mutants.
J Bacteriol  (1993)  175(22)   7247-53  
[PubMed ID = 8226671]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks
Request
page-top
/ecoli/strain