Gene Mutants - Detail

ME No. ME9247
Strain Name AQ3021
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype thr-1 leu-6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL-31 tsx-33 sup-37 thyA recF144
Parent Donor
Parent Recipient
Methods
Latest Marker
Selection
Source from A. J. Clark.
Reference Torrey TA, Kogoma T.
Genetic analysis of constitutive stable DNA replication in rnh mutants of Escherichia coli K12.
Mol Gen Genet  (1987)  208(3)   420-7  
[PubMed ID = 2823060]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks same as JC8931
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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