Gene Mutants - Detail

ME No. ME9244
Strain Name AQ2984
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype thi-1 his-4 proB48 argE3 lacI3 lacZ118 ara-14 galK2 xyl-5 mtl-1 recA200 recA254o srlC300::Tn10 rpsL31 supE44 tsx-33
Parent Donor
Parent Recipient
Methods
Latest Marker
Selection
Source
Reference Torrey TA, Kogoma T.
Genetic analysis of constitutive stable DNA replication in rnh mutants of Escherichia coli K12.
Mol Gen Genet  (1987)  208(3)   420-7  
[PubMed ID = 2823060]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml).
Other Remarks same as N1715: Lloyd R. G., 1983, M. G. G., 189, 157-161.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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