Gene Mutants - Detail

ME No. ME9241
Strain Name AQ2891
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype rha-5 recA200 srlC::Tn10 thyA6 deoC2 lacZ53 rpsL151 rin-15 zag::Tn10 rnhA224
Parent Donor AQ978
Parent Recipient AQ2657
Methods TD./PI
Latest Marker Ls, cSDR, rnhA224
Selection Tc-r
Source
Gene Mutants Detail Info
Reference Torrey TA, Kogoma T.
Genetic analysis of constitutive stable DNA replication in rnh mutants of Escherichia coli K12.
Mol Gen Genet  (1987)  208(3)   420-7  
[PubMed ID = 2823060]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml).
Other Remarks Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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