Gene Mutants - Detail

ME No. ME9238
Strain Name AQ2787
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 proB ilv metB1 thyA recA200 deoB (or deoC) rnhA224
Parent Donor AQ270
Parent Recipient AQ666
Methods CROSS
Latest Marker UV(Ts); recA200, Thy-; thyA
Selection His+
Source
Gene Mutants Detail Info
Reference Kogoma T, Barnard KG, Hong X.
RecA, Tus protein and constitutive stable DNA replication in Escherichia coli rnhA mutants.
Mol Gen Genet  (1994)  244(5)   557-62  
[PubMed ID = 8078483]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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