Gene Mutants - Detail

ME No. ME9236
Strain Name AQ2781
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 proB ilv metB1 deoB (or deoC)
Parent Donor AQ165(JC5519)
Parent Recipient AQ634
Methods TD./PI
Latest Marker UVr
Selection Thy+
Source
Gene Mutants Detail Info
Reference Magee TR, Kogoma T.
Requirement of RecBC enzyme and an elevated level of activated RecA for induced stable DNA replication in Escherichia coli.
J Bacteriol  (1990)  172(4)   1834-9  
[PubMed ID = 2180906]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks
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