Gene Mutants - Detail

ME No. ME9235
Strain Name AQ2696
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 argH thyA deoB (or deoC) proA3 metD88
Parent Donor AQ1993
Parent Recipient AQ685
Methods TD./PI
Latest Marker Rif-s, rpoB+
Selection Met+
Source
Gene Mutants Detail Info
Reference Kogoma T, Subia NL, von Meyenburg K.
Function of ribonuclease H in initiation of DNA replication in Escherichia coli K-12.
Mol Gen Genet  (1985)  200(1)   103-9  
[PubMed ID = 2993805]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks
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