Gene Mutants - Detail

ME No. ME9230
Strain Name AQ2591
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 metB1 argH thyA rpoB340 deoB (or deoC) metD88 Δlac-3 ilv recF143
Parent Donor AQ2200
Parent Recipient AQ1065
Methods TD./PI
Latest Marker UVs, recF143
Selection Tr(42C)
Source
Gene Mutants Detail Info
Reference Magee TR, Kogoma T.
Rifampin-resistant replication of pBR322 derivatives in Escherichia coli cells induced for the SOS response.
J Bacteriol  (1991)  173(15)   4736-41  
[PubMed ID = 1856169]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
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