Gene Mutants - Detail

ME No. ME9218
Strain Name AQ2288
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 proB(or proC) ilv metB1 thyA deoB(or deoC) recF322::Tn3 tnaA::Tn10
Parent Donor AQ2149(JC10990)
Parent Recipient AQ634
Methods TD./PI
Latest Marker UVs, recF322::Tn3
Selection Ap-r
Source
Gene Mutants Detail Info
Reference Subia NL, Kogoma T.
Concatemer formation of ColE1-type plasmids in mutants of Escherichia coli lacking RNase H activity.
J Mol Biol  (1986)  189(3)   389-99  
[PubMed ID = 3023625]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Request
page-top
/ecoli/strain