Gene Mutants - Detail

ME No. ME9211
Strain Name AQ2178
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 proB(or proC) ilv metB1 thyA deoB(or deoC) zig::Tn10 polA1
Parent Donor CM5280
Parent Recipient AQ634
Methods TD./PI
Latest Marker MMS-s, polA1
Selection Tc-r
Source
Gene Mutants Detail Info
Reference Bates DB, Asai T, Cao Y, Chambers MW, Cadwell GW, Boye E, Kogoma T.
The DnaA box R4 in the minimal oriC is dispensable for initiation of Escherichia coli chromosome replication.
Nucleic Acids Res  (1995)  23(16)   3119-25  
[PubMed ID = 7667087]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
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