Gene Mutants - Detail

ME No. ME9209
Strain Name AQ2152
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype thr-1 leuB6 thi-1 lacY1 galK2 ara-14 xyl-5 mtl-1 proA2 his-4 argE3 rpsL31 tsx-33 supE44 recA281o(Con) lexA3(Ind-) srlC300::Tn10
Parent Donor
Parent Recipient
Methods
Latest Marker
Selection
Source
Reference Magee TR, Kogoma T.
Requirement of RecBC enzyme and an elevated level of activated RecA for induced stable DNA replication in Escherichia coli.
J Bacteriol  (1990)  172(4)   1834-9  
[PubMed ID = 2180906]  [RRC reference
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks same as JC11867: Clark A. J., 1982, Biochimie, 64, 669-675.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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