Gene Mutants - Detail

ME No. ME9199
Strain Name AQ2020
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 ilv-192 proA3 argH metD88 asnB50::Tn5 thyA deoB/C dnaA5 rnhA224
Parent Donor TC743
Parent Recipient AQ1173
Methods TD./PI
Latest Marker Rif-s, rpoB+
Selection Met+
Source
Gene Mutants Detail Info
Reference Kogoma T, Subia NL, von Meyenburg K.
Function of ribonuclease H in initiation of DNA replication in Escherichia coli K-12.
Mol Gen Genet  (1985)  200(1)   103-9  
[PubMed ID = 2993805]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml).
Other Remarks Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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