Gene Mutants - Detail

ME No. ME9198
Strain Name AQ2016
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 ilv-192 proA3 metD88 asnB50::Tn5 thyA deoB/C dnaA5
Parent Donor AQ1993
Parent Recipient AQ1175
Methods TD./PI
Latest Marker Rif-s, rpoB+
Selection Met+
Source
Gene Mutants Detail Info
Reference Kogoma T, Subia NL, von Meyenburg K.
Function of ribonuclease H in initiation of DNA replication in Escherichia coli K-12.
Mol Gen Genet  (1985)  200(1)   103-9  
[PubMed ID = 2993805]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), kanamycin (55μg/ml).
Other Remarks
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