Gene Mutants - Detail

ME No. ME9596
Strain Name AQ10601
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype trpA9605 his-29 pro ilv metB1 thyA deoB (or deoC) Δ(lacIPOZY)x74 proC::Tn5 lexA3 malF3089::Tn10
Parent Donor AQ8300
Parent Recipient AQ9504
Methods TD./PI
Latest Marker UVs, lexA3
Selection Tc-r
Source
Gene Mutants Detail Info
Reference Hong X, Cadwell GW, Kogoma T.
Activation of stable DNA replication in rapidly growing Escherichia coli at the time of entry to stationary phase.
Mol Microbiol  (1996)  21(5)   953-61  
[PubMed ID = 8885266]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (50μg/ml), His (50μg/ml), Ile (50μg/ml), Val (50μg/ml) Met (50μg/ml), Pro (40μg/ml), Arg (50μg/ml), Asn (50μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Further information: Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
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