Gene Mutants - Detail

ME No. ME9275
Strain Name AQ3589
Sex F-
Plasmid Integrated
Plasmid Autonomous
Prophage
Genotype metE90 trpA9605 lacI22 lacZ118 rpsL171 azi-9 nalA18 thyA708 deo-29 rnhA102 recA200 rin-15 tnaA::Tn10 dnaA5
Parent Donor AQ1103
Parent Recipient AQ527
Methods TD./PI
Latest Marker Ls, dnaA5
Selection Tc-r
Source
Gene Mutants Detail Info
Reference Torrey TA, Kogoma T.
Genetic analysis of constitutive stable DNA replication in rnh mutants of Escherichia coli K12.
Mol Gen Genet  (1987)  208(3)   420-7  
[PubMed ID = 2823060]  [RRC reference] 
Culture Condition Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Other Remarks Ls: Srm phenotype, sensitivity for rich media. Ref: Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465. Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238.
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